plot 2000 version 6.10 Search Results


jasco 880 pu hplc apparatus  (JASCO Inc)
97
JASCO Inc jasco 880 pu hplc apparatus
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
Jasco 880 Pu Hplc Apparatus, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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uv vis md 910 pda detector  (JASCO Inc)
97
JASCO Inc uv vis md 910 pda detector
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
Uv Vis Md 910 Pda Detector, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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shelxtl v.6.10  (Bruker Corporation)
90
Bruker Corporation shelxtl v.6.10
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
Shelxtl V.6.10, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sigmaplot 2000 windows  (SPSS Inc)
90
SPSS Inc sigmaplot 2000 windows
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
Sigmaplot 2000 Windows, supplied by SPSS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p-2000 puller  (Sutter Instrument Company)
90
Sutter Instrument Company p-2000 puller
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
P 2000 Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lipofectamine 2000  (Thermo Fisher)
86
Thermo Fisher lipofectamine 2000
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
Lipofectamine 2000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nrecon software  (Bruker Corporation)
90
Bruker Corporation nrecon software
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
Nrecon Software, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti gfp  (Rockland Immunochemicals)
93
Rockland Immunochemicals rabbit anti gfp
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
Rabbit Anti Gfp, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ir800 conjugated donkey anti mouse igg  (Rockland Immunochemicals)
93
Rockland Immunochemicals ir800 conjugated donkey anti mouse igg
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
Ir800 Conjugated Donkey Anti Mouse Igg, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti mouse antibody  (Rockland Immunochemicals)
96
Rockland Immunochemicals anti mouse antibody
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
Anti Mouse Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti rabbit igg h l  (Rockland Immunochemicals)
95
Rockland Immunochemicals goat anti rabbit igg h l
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
Goat Anti Rabbit Igg H L, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tritc-labeled donkey anti-mouse antibody  (Rockland Immunochemicals)
90
Rockland Immunochemicals tritc-labeled donkey anti-mouse antibody
Fig. 1. <t>HPLC</t> profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.
Tritc Labeled Donkey Anti Mouse Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 1. HPLC profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.

Journal: European journal of biochemistry

Article Title: The N-glycans of jack bean alpha-mannosidase. Structure, topology and function.

doi: 10.1046/j.1432-1327.1999.00598.x

Figure Lengend Snippet: Fig. 1. HPLC profiles of PA-sugar chains from jack bean a-man- nosidase. Panel A: RP-HPLC of PA-derivatives from jack bean a-man- nosidase. The PA-derivatives were applied onto a Cosmosil 5C18-AR (6.0 250 mm) column and eluted as described in the text. The run- through-fraction (around 9 min) contained some PA-derivatives bearing only one GlcNAc residue at their reducing-ends, which are known to be derived from released intact N-glycans as by-products of hydrazinolysis [7,12]. Panel B: Size-fractionation HPLC of the partially purified PA-sugar chains shown in Panel A (Peaks I and II) by RP-HPLC.

Article Snippet: The two glycopeptide fractions [elution volume from 244±292 mL (high-mannose-type glycan) and from 408±500 mL (complex-type glycan)] were concentrated in a rotary evaporator and the former glycopeptide-fraction was applied to a Jasco 880-PU HPLC apparatus with a Poros R1 column (4.6 100 mm).

Techniques: Residue, Derivative Assay, Fractionation, Purification

Fig. 3. RP-HPLC of a lysylendopeptidase digest of alkylated jack bean a-mannosidase. The lysylendopeptidase digest of alkylated jack bean a- mannosidase (100 mg) was applied onto a Vydac C8 column. The column was eluted by a linear gradient of 2±40% acetonitrile in 0.1% trifluoroacetic acid. Panel A: Total ion monitoring chromatogram (TIC). `RT: 28.2 min' indicates the position of glycopeptide (GP) which eluted after 28.2 min. Panel B: Precursor ion (m/z 366.0; Hex-HexNAc) monitoring. Panel C: Precursor ion (m/z 204.1; HexNAc) monitoring. Panel D: ESI-MS spectrum of the glycopeptide GP (complex-type) from Panel A.

Journal: European journal of biochemistry

Article Title: The N-glycans of jack bean alpha-mannosidase. Structure, topology and function.

doi: 10.1046/j.1432-1327.1999.00598.x

Figure Lengend Snippet: Fig. 3. RP-HPLC of a lysylendopeptidase digest of alkylated jack bean a-mannosidase. The lysylendopeptidase digest of alkylated jack bean a- mannosidase (100 mg) was applied onto a Vydac C8 column. The column was eluted by a linear gradient of 2±40% acetonitrile in 0.1% trifluoroacetic acid. Panel A: Total ion monitoring chromatogram (TIC). `RT: 28.2 min' indicates the position of glycopeptide (GP) which eluted after 28.2 min. Panel B: Precursor ion (m/z 366.0; Hex-HexNAc) monitoring. Panel C: Precursor ion (m/z 204.1; HexNAc) monitoring. Panel D: ESI-MS spectrum of the glycopeptide GP (complex-type) from Panel A.

Article Snippet: The two glycopeptide fractions [elution volume from 244±292 mL (high-mannose-type glycan) and from 408±500 mL (complex-type glycan)] were concentrated in a rotary evaporator and the former glycopeptide-fraction was applied to a Jasco 880-PU HPLC apparatus with a Poros R1 column (4.6 100 mm).

Techniques: Glycoproteomics

Fig. 5. RP-HPLC of a glycopeptide-fraction obtained by gel-filtration of a lysylendopeptidase-digest of alkylated jack bean a-mannosidase. The glycopeptide fraction obtained by Sephadex G-25 gel-filtration (fraction volume from 244 to 292 mL) was applied onto a Poros R1 column. The column was eluted with 5% acetonitrile in 0.1% trifluoroacetic acid for 5 min, followed by a linear gradient of 5±60% acetonitrile in 0.1% trifluoroacetic acid. Only the peak indicated by an arrow was found to contain neutral sugars. GP (high-mannose-type) indicates a glycopeptide bearing the high-mannose-type N-glycan.

Journal: European journal of biochemistry

Article Title: The N-glycans of jack bean alpha-mannosidase. Structure, topology and function.

doi: 10.1046/j.1432-1327.1999.00598.x

Figure Lengend Snippet: Fig. 5. RP-HPLC of a glycopeptide-fraction obtained by gel-filtration of a lysylendopeptidase-digest of alkylated jack bean a-mannosidase. The glycopeptide fraction obtained by Sephadex G-25 gel-filtration (fraction volume from 244 to 292 mL) was applied onto a Poros R1 column. The column was eluted with 5% acetonitrile in 0.1% trifluoroacetic acid for 5 min, followed by a linear gradient of 5±60% acetonitrile in 0.1% trifluoroacetic acid. Only the peak indicated by an arrow was found to contain neutral sugars. GP (high-mannose-type) indicates a glycopeptide bearing the high-mannose-type N-glycan.

Article Snippet: The two glycopeptide fractions [elution volume from 244±292 mL (high-mannose-type glycan) and from 408±500 mL (complex-type glycan)] were concentrated in a rotary evaporator and the former glycopeptide-fraction was applied to a Jasco 880-PU HPLC apparatus with a Poros R1 column (4.6 100 mm).

Techniques: Glycoproteomics, Filtration